Іntensity of oxygen consumption by bull sperm due to the action of L-carnitine
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Дата
2017
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Анотація
The research is devoted to the study of peculiarities of oxygen
consumption intensity and the activity of enzymes of antioxidant
protection of bull sperm due to the action of L-carnitine. We used
bull sperm that was mixed with sperm dilution for the “Bioexel”
to the ratio 1:1. Diluted semen was divided into two parts – one
control and three experimental. L-carnitine was added to the diluent in the amount of 10 mg/100 ml in part 2, 30 mg/100 ml in
part 3, and 60 mg/100 ml in part 4; the sperm was dissolved in
this liquid to the ratio of 3:1. The intensity of oxygen consumption, the activity of enzymes superoxidedismutase, catalase, glutathioneperoxidase and survival of spermatozoa were thoroughly
investigated in the diluted semen. L-carnitine, which was added
to the diluent of semen 10 and 30 mg/100 ml, stimulates aerobic
glycolysis by 5.9 and 11.9%, and decreases oxidative processes by
5.6% and 11.4%, respectively. It is not related to the synthesis of
ATP. It increases the survival of bull spermatozoa. The results of
current research show that carnitine promotes a rise in the activity of CAT and GPO, and the activity of SOD decreases. We
established that L-carnitine impacts the reduction of the overall
consumption of oxygen by increasing the share of mitochondrial
oxygen consumption and aerobic glycolysis, along with the decrease in free radical oxidation. It is probably related to the metabolic activation between the semen and the environment. We
found that the optimal concentration of L-carnitine in the bull
diluted sperm is 30 mg/100 ml.
Опис
Ключові слова
oxygen consumption, antioxidant enzymes, L-carnitine, semen, bulls